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Genomic Division

The Genomic Division supports the other divisions, in respect to any gene of interest, by isolating defined DNA sequences, obtaining multiple copies of it in vivo, so that the sequence may be expressed in a particular host system.

Presently, we are using two systems i.e. Escherichia coli and Pichia pastoris for heterologous expression of certain gene. PCR and artificial gene synthesis based techniques are also used to isolate the gene of interest. In order to express certain coding sequence in the system, T7 expression system pET and pPIC based vector for E. coli and P. pastoris expression system will be used respectively.

The specific objectives of this division are:

  • Genomic or synthesizing a particular nucleotide sequence of interest.
  • Preparing recombinant DNA construct.
  • Expressing full length or partially DNA sequence in prokaryotic and eukaryotic cells.
  • Performing molecular analysis at DNA, RNA, and Protein level.

The division is equipped with basic DNA molecular hardware such as Thermocycler (real-time PCR and T-gradient PCR) and Electroporator.

Our current work is on cloning and expression of human AFP. AFP is a molecule produced in the developing embryo and fetus. The AFP gene is located at chromosome 4 in the human genome and the protein can be divided into three domains. AFP in adults is often associated with hepatoma or teratoma. This protein is a well recognized tumor marker for HCC. Currently, we are working on the protein expression of three separate domains in hAFP using E. coli and P. pastoris expression systems. The goal is to produce glycosylated and non-glycosylated hAFP domain for immune response study.

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